Friday, June 13, 2014

Effect of mast cell mediators on sleep

I have always been tired.  For several years, it was easy to attribute it to the fact that I was always working or in school.  After I graduated and got a 40-hour a week job, it became obvious that my tiredness was a symptom.  Even still, I struggled to control it.  It felt like if I could manage this one aspect of my life, everything else would fall into place. 

Any time I would get into a reasonable rhythm, something would change and I would have to start all over again.  Other symptoms have been harder to deal with in some ways, but it is safe to say that being so tired has caused me more grief than anything else.  It is the root cause of all the myriad ways I have lost control over my life.
I am now in a really obnoxious cycle in which I am awake for 30 hours and then sleep for 18 hours.  It is really disruptive and disorienting.  A few weeks ago, shortly after I was released from the hospital, I decided that I would no longer try to control my sleep.  I would sleep when my body wanted to sleep, because it wasn’t worth how sick I felt when I forced myself to wake even when I was exhausted.  So I gave up and moved on.  Some things are just not worth fighting after so long.
Last night while I couldn’t sleep, I did some research on why I couldn’t sleep and wrote it up for all of you mast cell kids who also can’t sleep.  SPOILER ALERT: It’s because of mast cells.
Mast cells are found in several parts of the brain.  Just like in other places, they degranulate or secrete factors, and they influence activity of the central nervous system.  One of the ways they affect the body is by participating in the sleep/wake cycle.
About half of the histamine found in the brain is released by mast cells.  Histamine actually increases wakefulness and decreases sleepiness.  This has been addressed in multiple papers, but one of the most well designed experiments involved mast cell deficient mice.  These mice were much more tired than the mice who had normal amounts of mast cells.
Histamine activity in the brain is increased during food deprivation or starvation conditions.  This is part of why eating regularly is important to sleep hygiene, even in the typical population.  When you’re hungry at bedtime, your mast cells tell your brain that it needs to find food that it doesn’t starve.
This same study also showed that the number of mast cells in the brain is determined, at least in part, but stress levels.  Chronic fight or flight type of stress, like exposure to a predator, increased the number of mast cells.  This makes sense because you need to stay awake to fight.  Social stress, like isolation, decreased the number of mast cells.
Treatment with H1 blocking antihistamines (Benadryl, etc) decreased wakefulness.  This indicates that the sleepiness might be due to preventing the release of histamine from mast cells.  Conversely, treatment with an H3 blocker actually made you less tired.  (I can attest to the fact that ketotifen, an H3 blocker, does in fact make me less tired.)  Treatment with substances that prevented histamine synthesis caused sleepiness.
Prostaglandin D2, another mast cell released factor, has been studied a lot as a regulator in the sleep/wake cycle.  Infusion of PGD2 into the brains of mice causes large and sustained increases in sleep. 
PGD2 activates cells through DP1 receptors.  When these receptors are stimulated, adenosine is released into the brain.  Adenosine increases sleepiness.  Mild to moderate levels of inflammation cause increased production and secretion of PGD2, resulting in tiredness.  However, in severe inflammation, another molecule, PGE2, is secreted preferentially by other cells and stimulates mast cells to release histamine.  This might explain why people who are chronically ill sleep so poorly despite being so tired. Sleep in people with high PGE2 is fragmented and not deep to the same extent as the general population. 
References:
Saper, Clifford, et al.  Neural circuitry engaged by prostaglandins during the sickness syndrome.  Nature Neuroscience 15, 1088-1095 (2012)
Chikahisa S, Kodama T, Soya A, Sagawa Y, Ishimaru Y, et al. (2013) Histamine from Brain Resident MAST Cells Promotes Wakefulness and Modulates Behavioral States. PLoS ONE 8(10): e78434. doi:10.1371/journal.pone.0078434

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